human tdg gene Search Results


gfp tdg  (Sino Biological)
91
Sino Biological gfp tdg
<t>TDG</t> forms condensates in cells. A , representative confocal fluorescent microscopy images of HeLa cells transfected with TDG fused to <t>GFP.</t> Red box indicates a larger TDG condensate in the nucleolus. Scale bars represent 5 μm. B , diameter of individual chromatin droplets formed by TDG in live HeLa cells. Data are mean ± SD (nucleoplasm: n = 10 cells, nucleolus: n = 3 cells). C , confocal fluorescent microscopy images and FRAP curve of GFP-TDG in HeLa cells. Data are mean ± SD (nucleoplasm: n = 10 cells, nucleolus: n = 3 cells). Scale bars represent 5 μm. D , representative fluorescent microscopy images of endogenous TDG showing loss of TDG foci upon 1,6-HD treatment. TDG was detected by immunostaining-fixed cells with TDG-specific antibody. Scale bars represent 5 μm. E , quantification of foci number and intensity upon of TDG with either 2,5-HD or 1,6-HD. Foci number are mean ± SD (n = 3 cells) for pre- and post-10 min treatment. Foci intensity is mean ± SD for 464, 428, and 335 individual foci pre-, post-2,5-HD, and post-1,6-HD treatment, respectively. ∗∗ p < 0.01; ∗∗∗ p < 0.001; and ∗∗∗∗ p < 0.0001. 1,6-HD, 1,6-hexanediol; 2,5-HD, 2,5-hexanediol; FRAP, fluorescence recovery after photobleaching; TDG, thymine DNA glycosylase.
Gfp Tdg, supplied by Sino Biological, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+tdg+gene/pmc10404674-213-4-11?v=Sino+Biological
Average 91 stars, based on 1 article reviews
gfp tdg - by Bioz Stars, 2026-07
91/100 stars
  Buy from Supplier
human tdg gene orf cdna clone expression plasmid  (Sino Biological)
91
Sino Biological human tdg gene orf cdna clone expression plasmid
<t>TDG</t> forms condensates in cells. A , representative confocal fluorescent microscopy images of HeLa cells transfected with TDG fused to <t>GFP.</t> Red box indicates a larger TDG condensate in the nucleolus. Scale bars represent 5 μm. B , diameter of individual chromatin droplets formed by TDG in live HeLa cells. Data are mean ± SD (nucleoplasm: n = 10 cells, nucleolus: n = 3 cells). C , confocal fluorescent microscopy images and FRAP curve of GFP-TDG in HeLa cells. Data are mean ± SD (nucleoplasm: n = 10 cells, nucleolus: n = 3 cells). Scale bars represent 5 μm. D , representative fluorescent microscopy images of endogenous TDG showing loss of TDG foci upon 1,6-HD treatment. TDG was detected by immunostaining-fixed cells with TDG-specific antibody. Scale bars represent 5 μm. E , quantification of foci number and intensity upon of TDG with either 2,5-HD or 1,6-HD. Foci number are mean ± SD (n = 3 cells) for pre- and post-10 min treatment. Foci intensity is mean ± SD for 464, 428, and 335 individual foci pre-, post-2,5-HD, and post-1,6-HD treatment, respectively. ∗∗ p < 0.01; ∗∗∗ p < 0.001; and ∗∗∗∗ p < 0.0001. 1,6-HD, 1,6-hexanediol; 2,5-HD, 2,5-hexanediol; FRAP, fluorescence recovery after photobleaching; TDG, thymine DNA glycosylase.
Human Tdg Gene Orf Cdna Clone Expression Plasmid, supplied by Sino Biological, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+tdg+gene/custom%40hg13000-ut%4010%2E1101%2F2025%2E08%2E05%2E668694?v=Sino+Biological
Average 91 stars, based on 1 article reviews
human tdg gene orf cdna clone expression plasmid - by Bioz Stars, 2026-07
91/100 stars
  Buy from Supplier
human tdg gene orf cdna clone in cloning vector  (Sino Biological)
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Full length Clone DNA of Human thymine DNA glycosylase
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thymine dna glycosylase tdg human gene knockout kit  (OriGene)
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TDG KN2 0 Human gene knockout kit via CRISPR non homology mediated
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Image Search Results


TDG forms condensates in cells. A , representative confocal fluorescent microscopy images of HeLa cells transfected with TDG fused to GFP. Red box indicates a larger TDG condensate in the nucleolus. Scale bars represent 5 μm. B , diameter of individual chromatin droplets formed by TDG in live HeLa cells. Data are mean ± SD (nucleoplasm: n = 10 cells, nucleolus: n = 3 cells). C , confocal fluorescent microscopy images and FRAP curve of GFP-TDG in HeLa cells. Data are mean ± SD (nucleoplasm: n = 10 cells, nucleolus: n = 3 cells). Scale bars represent 5 μm. D , representative fluorescent microscopy images of endogenous TDG showing loss of TDG foci upon 1,6-HD treatment. TDG was detected by immunostaining-fixed cells with TDG-specific antibody. Scale bars represent 5 μm. E , quantification of foci number and intensity upon of TDG with either 2,5-HD or 1,6-HD. Foci number are mean ± SD (n = 3 cells) for pre- and post-10 min treatment. Foci intensity is mean ± SD for 464, 428, and 335 individual foci pre-, post-2,5-HD, and post-1,6-HD treatment, respectively. ∗∗ p < 0.01; ∗∗∗ p < 0.001; and ∗∗∗∗ p < 0.0001. 1,6-HD, 1,6-hexanediol; 2,5-HD, 2,5-hexanediol; FRAP, fluorescence recovery after photobleaching; TDG, thymine DNA glycosylase.

Journal: The Journal of Biological Chemistry

Article Title: Thymine DNA glycosylase mediates chromatin phase separation in a DNA methylation–dependent manner

doi: 10.1016/j.jbc.2023.104907

Figure Lengend Snippet: TDG forms condensates in cells. A , representative confocal fluorescent microscopy images of HeLa cells transfected with TDG fused to GFP. Red box indicates a larger TDG condensate in the nucleolus. Scale bars represent 5 μm. B , diameter of individual chromatin droplets formed by TDG in live HeLa cells. Data are mean ± SD (nucleoplasm: n = 10 cells, nucleolus: n = 3 cells). C , confocal fluorescent microscopy images and FRAP curve of GFP-TDG in HeLa cells. Data are mean ± SD (nucleoplasm: n = 10 cells, nucleolus: n = 3 cells). Scale bars represent 5 μm. D , representative fluorescent microscopy images of endogenous TDG showing loss of TDG foci upon 1,6-HD treatment. TDG was detected by immunostaining-fixed cells with TDG-specific antibody. Scale bars represent 5 μm. E , quantification of foci number and intensity upon of TDG with either 2,5-HD or 1,6-HD. Foci number are mean ± SD (n = 3 cells) for pre- and post-10 min treatment. Foci intensity is mean ± SD for 464, 428, and 335 individual foci pre-, post-2,5-HD, and post-1,6-HD treatment, respectively. ∗∗ p < 0.01; ∗∗∗ p < 0.001; and ∗∗∗∗ p < 0.0001. 1,6-HD, 1,6-hexanediol; 2,5-HD, 2,5-hexanediol; FRAP, fluorescence recovery after photobleaching; TDG, thymine DNA glycosylase.

Article Snippet: The expression vector for GFP-TDG (catalog no.: HG13000-ANG) was purchased from Sino Biological.

Techniques: Microscopy, Transfection, Immunostaining, Fluorescence